Sensitivity and reliability of new indicators for diagnosing neuromyelitis optica.
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Immunofluorescence of the human aquaporin-4-M23-mCherry V79 cells. OAPs: Orthogonal arrays of anti-aquaporin-4 autoantibodies particles. | |
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Neuromyelitis optica is a neurological autoimmune demyelinating disease that usually involves severe optic neuritis and transverse, longitudinally extensive myelitis. Routine methods for diagnosing neuromyelitis optica are mainly dependent on patient’s clinical symptoms and detection of serum autoantibodies, which is lower sensitive. Miao Li and colleagues reported a related study, named “Detection of anti-aquaporin-4 autoantibodies in the sera of Chinese neuromyelitis optica patients”, which was published in the Neural Regeneration Research (Vol. 8, No. 8, 2013). Results from this study showed that Chinese hamster lung fibroblast (V79) cells transfected with a human aquaporin-4-mCherry fusion protein gene were used to detect anti-aquaporin-4 antibody in neuromyelitis optica patient sera by immunofluorescence. Anti-aquaporin-4 autoantibody was stably detected by immunofluorescence in neuromyelitis optica patient sera exclusively. The sensitivity of the assay for neuromyelitis optica was 90% and the specificity for neuromyelitis optica was 100%. A positive correlation was detected between Expanded Disability Status Scale scores and serum anti-aquaporin-4 antibody titers in neuromyelitis optica patient sera. Authors believed that the anti-aquaporin-4 antibody assay is highly sensitive and specific in the sera of Chinese neuromyelitis optica patients. Detection of aquaporin-4 autoantibody is important for the diagnosis and treatment of neuromyelitis optica.
Article: ” Detection of anti-aquaporin-4 autoantibodies in the sera of Chinese neuromyelitis optica patients,” by Miao Li1, 2, Weiheng Su3, Jie Wang2, Francesco Pisani4, Antonio Frigeri4, Tonghui Ma1 (1 Membrane Channel Research Laboratory, Northeast Normal University, Changchun 130024, Jilin Province, China; 2 Department of Neurosurgery, China-Japan Union Hospital, Jilin University, Changchun 130033, Jilin Province, China; 3 National Engineering Laboratory for AIDS Vaccine, College of Life Science, Jilin University, Changchun 130012, Jilin Province, China; 4 Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics (CEGBA), University of Bari, I-70126 Bari, Italy)
Li M, Su WH, Wang J, Pisani F, Frigeri A, Ma TH. Detection of anti-aquaporin-4 autoantibodies in the sera of Chinese neuromyelitis optica patients. Neural Regen Res. 2013;8(8):708-713.