Development of a cell-based assay for the det… [J Neuroimmunol. 2014] – PubMed – NCBI.

J Neuroimmunol. 2014 Aug 15;273(1-2):103-10. doi: 10.1016/j.jneuroim.2014.06.003. Epub 2014 Jun 12.

Development of a cell-based assay for the detection of anti-aquaporin 1 antibodies in neuromyelitis optica spectrum disorders.

Long Y¹, Zheng Y¹, Shan F¹, Chen M¹, Fan Y¹, Zhang B¹, Gao C², Gao Q¹, Yang N³.

Author information

¹Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China.
²Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and the Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China; Department of Neurology, The Second Affiliated Hospital of GuangZhou Medical University, 250# Changgang East Road, GuangZhou, 510260 Guangdong Province, China. Electronic address: smilegaocong@126.com.
³Department of Neurology, The Fifth Affiliated Hospital of GuangZhou Medical University, 621# Gangwan Road, GuangZhou, 510700 Guangdong Province, China.

Abstract

OBJECTIVE:

To develop a cell-based assay (CBA) to detect aquaporin 1 (AQP1) antibodies and determine sensitivity/specificity in patients with neuromyelitis optica (NMO) spectrum disorders.

METHODS:

A HEK-293T transfected cell model expressing AQP1 was established and detected to be serum AQP1 antibodies.

RESULTS:

AQP1 antibodies were present in 73/98 (74.5%) AQP4 antibody-positive patients. Some AQP4 antibody-negative patients were also AQP1 antibody-positive. Test sensitivity was 74.5% in 98 AQP4 antibody-positive patients. Test specificity was 79.6% in 67 multiple sclerosis (MS) patients and 31 controls.

CONCLUSION:

A sensitive and simple CBA was developed to detect serum AQP1 antibodies. AQP1 antibodies were mainly present in NMO and its high-risk syndrome, but also in some MS patients.

KEYWORDS:

Aquaporin 1 antibody; Cell-based assay; Multiple sclerosis; Neuromyelitis optica

PMID:: 24972850; [PubMed – in process]

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Development of a cell-based assay for the det… [J Neuroimmunol. 2014] – PubMed – NCBI